Antiferritin single-chain antibody: a functional protein with incomplete folding?
نویسندگان
چکیده
منابع مشابه
Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting.
The complementary determining regions (CDRs) from the fluorescein-binding antibody 4-4-20, which yields almost no soluble protein in periplasmic expression in Escherichia coli, were transplanted to the framework of the humanized antibody 4D5. The resulting single-chain Fv fragment (scFv) 4D5Flu showed both a dramatic improvement in soluble expression, even at 37 degrees C, and an improved therm...
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Antibodies are proteins with the ability to bind antigens rapidly and specifically. Antibodies consist of several different parts. The complementary determining regions (CDR) are the parts that recognize the antigen are the focus of this study. The aims of this study were to synthesize and produce a single-chain antibody (scFv) of the anti-cytokeratin 8 monoclonal IgG antibody TS1 and to functi...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1998
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(98)01601-9